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collagenase type ii  (Thermo Fisher)


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    Structured Review

    Thermo Fisher collagenase type ii
    Collagenase Type Ii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagenase+type+ii/Collagenase/pmc12809746-140-38-41
    Average 97 stars, based on 1 article reviews
    collagenase type ii - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids
    Article Snippet: .. For enzymatic dissociation, liver tissues were incubated in enzyme solution containing 0.125 mg mL −1 collagenase type XI and 0.125 mg mL −1 dispase II for 45 min, while lung tissues were incubated in 2 mg mL −1 collagenase type II (Thermo Fisher Scientific) for 1 h. Both digestions were carried out at 37 °C with gentle shaking. ..

    Article Title: Exploring bone formation mechanism and pattern during RANKL inhibition in a fibrous dysplasia mouse model.
    Article Snippet: .. Briefly, after removal of skin and tendons, tail vertebrae were placed in HBSS (Gibco, Thermo Fisher Scientific) with 2 mg/ml collagenase type II (Gibco, Thermo Fisher Scientific) and incubated in a water bath for 15 minutes at 200 relative centrifugal force (rcf), to remove the outer layer of periosteum. ..

    Gentle:

    Article Title: Esophagus extracellular matrix with microenvironmental complexity for esophageal organoids
    Article Snippet: .. For enzymatic dissociation, liver tissues were incubated in enzyme solution containing 0.125 mg mL −1 collagenase type XI and 0.125 mg mL −1 dispase II for 45 min, while lung tissues were incubated in 2 mg mL −1 collagenase type II (Thermo Fisher Scientific) for 1 h. Both digestions were carried out at 37 °C with gentle shaking. ..

    Sterility:

    Article Title: Radiation-grafted poly(acrylic acid) on bacterial nanocellulose scaffolds modulates human adipose-derived stem cell morphology, proliferation, and extracellular matrix expression.
    Article Snippet: .. The tissue was then minced with sterile surgical scissors and digested in a solution of collagenase type II (Gibco, 171001015 [3 mg/mL] in DMEM/F12 at 37 ◦C for 45 min under constant agitation at 200 rpm. .. Enzymatic digestion was stopped by adding 1 mL fetal bovine serum (FBS; Gibco, 16000044), followed by 5 min of incubation with agitation.

    MTT Assay:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    RNA Extraction:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Isolation:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    In Vivo:

    Article Title: Sunitinib induces immunogenic cell death through eIF2α phosphorylation to potentiate immunotherapy in HCC
    Article Snippet: .. For analysis of immune cell populations in vivo , minced tumors were enzymatically digested by a cocktail of 5 mg/mL Dispase II (Cat#42613-33-2, Sigma-Aldrich), 2.5 mg/mL Collagenase D (Cat#11088866001, Sigma-Aldrich), and 2.5 mg/mL Collagenase type II (Cat# 07419, Stemcell) in PBS (Cat#10010023, Gibco). ..

    other:

    Article Title: FGF21 targets pathways which enhance insulin sensitivity in brown adipose, but not skeletal muscle in mice.
    Article Snippet: A total of 2 micrograms RNA from each sample 9 was used to generate cDNA (High-Capacity cDNA Reverse Transcription Kit; Life Technologies), 10 and QPCR was conducted using SYBR green (Invitrogen).

    Article Title: FGF21 targets pathways which enhance insulin sensitivity in brown adipose but not skeletal muscle in mice
    Article Snippet: RNA was isolated from the indicated tissues following Trizol (Invitrogen) protocol.



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